Sex Identification in the Canary Using Dna Typing Methods

In many bird species, the sex determination is very difficult in young birds and many adults. Nowadays, sex identification of animals throughout their lives is possible by molecular genetic techniques. In the present study, the sex identification of canaries by PCR methods based on chromo helicase DNA binding protein CHD-Z and CHD-W genes were explained. Genomic DNA was extracted from feather bulb (old and young) of canary. PCR reaction was performed by a set of novel primers. The results showed two amplified fragments, about 345 and 306 bp for female, and only one 306 bp amplified PCR product for male canaries. This result was shown for each sample. This sex identification system which presented for the first time, especially in canaries. We have established a fast, safe, accurate and inexpensive procedure for sex typing of canary using DNA extracted from feathers.